GENETIC ENGINEERING OF COTTON WITH A NOVEL CRY2AX1 GENE TO IMPART INSECT RESISTANCE AGAINST HELICOVERPA ARMIGERA

Genetic engineering of cotton with a novel cry2AX1 gene to impart insect resistance against Helicoverpa armigera

Embryogenic calli of cotton (Coker310) were cocultivated with the Agrobacterium tumefaciens strain LBA4404 harbouring the codon-optimised, chimeric cry2AX1 gene consisting of sequences from cry2Aa and cry2Ac genes isolated from Indian fr5945 strains of Bacillus thuringiensis.Forty-eight putative transgenic plants were regenerated, and PCR analysis

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Analysis of Traditional Medical Disputes: Data from the Korean Acupuncture and Moxibustion Medicine Society (2013-2017)

Background The purpose of this study was to analyze the medical dispute cases in Acupuncture and Moxibustion treatment, and present the guidelines of treatment to prevent medical disputes.Methods Korean Acupuncture and Moxibustion Medicine Society medical dispute cases from January 2013 to September 2018 were collected and analyzed.Results There we

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Application of targeted high-throughput sequencing as a diagnostic tool for neonatal genetic metabolic diseases following tandem mass spectrometry screening

BackgroundTandem mass spectrometry (MS/MS) is a crucial technique for detecting inborn errors of metabolism (IEM) in newborns.However, the high false positive rate poses challenges in diagnosing specific types of diseases.Therefore, this study aimed to evaluate the role of targeted next-generation sequencing (NGS) in the accurate diagnosis of posit

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